期刊
JOURNAL OF NEUROSCIENCE METHODS
卷 135, 期 1-2, 页码 95-105出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jneumeth.2003.12.022
关键词
tracking neurons; tetrode; electrode drift; extracellular recording
资金
- NEI NIH HHS [R01-EY13595] Funding Source: Medline
Tetrodes allow isolation Of Multiple neurons at a single recording site by clustering spikes. Due to electrode drift and perhaps due to time-varving neuronal properties. positions and shapes of clusters change in time. As data is typically collected in sequential files, to track neurons across files one has to decide which clusters from different files belong to the same neuron. We report on a semi-automated neuron tracking procedure that uses computed similarities between the mean spike waveforms of the clusters. The clusters with the most similar waveforms are assigned to the same neuron, provided their similarity exceeds a threshold. To set this threshold, we calculate two distributions: of within-file similarities. and of best matches in the across adjacent file similarities. The threshold is set to the value that optimally separates the two distributions. We compare different measures of similarity (metrics) by their ability to separate these distributions. We find that these metrics do not differ drastically in their performance, but that taking into account the cross-channel noise Correlation significantly improves performance of all metrics. We also demonstrate the method on an independent dataset and show that neurons, as assigned by the procedure, have consistent physiological properties across files. (C) 2004 Elsevier B.V. All rights reserved.
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