期刊
ELECTROPHORESIS
卷 25, 期 12, 页码 1895-1900出版社
WILEY
DOI: 10.1002/elps.200305818
关键词
capillary array electrophoresis immunoassay; miniaturization; 2,4,6-trinitrotoluene
A high-throughput homogeneous immunoassay for the sensitive detection of 2,4,6-tri-nitrotoluene (TNT) has been developed using radial capillary array electrophoresis microdevices. Samples consisting of equilibrium mixtures of anti-TNT antibody (Ab), fluorescein-labeled TNT, and various concentrations of unlabeled TNT were electrokinetically injected into 48 channels of a radial capillary array electrophoresis microchannel plate. The rapid electrophoretic separation allows us to analyze the equilibrium ratio formed by the competition between the labeled and the unlabeled TNT for Ab binding. The simultaneous parallel TNT separations facilitate determination of a calibration curve for the TNT assay, which has high sensitivity (LOD, 1 ng/mL) and a wide dynamic range (1-300 ng/mL).
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