期刊
FEBS LETTERS
卷 567, 期 1, 页码 35-41出版社
WILEY
DOI: 10.1016/j.febslet.2004.03.066
关键词
RNA replication; RNA rcombination; Q beta replicase; molecular colonies; RNA world; molecular diagnostics
This paper summarizes results of the 40-year studies on replication and recombination of RNA molecules in the cell-free amplification system of bacteriophage Q. Special attention is paid to the molecular colony technique that has provided for the discovery of the nature of spontaneous RNA synthesis by Q replicase and of the ability of RNA molecules to spontaneously rearrange their sequences under physiological conditions. Also discussed is the impact of these data on the concept of RNA World and on the development of new in vitro cloning and diagnostic tools. (C) Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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