期刊
AQUACULTURE NUTRITION
卷 19, 期 1, 页码 45-53出版社
WILEY-HINDAWI
DOI: 10.1111/j.1365-2095.2011.00934.x
关键词
antioxidant; enterocyte; growth; intestine; Jian carp (Cyprinus carpio var; Jian); myo-inositol
类别
资金
- National Science Foundation of China [31001116]
- Program for New Century Excellent Talents in University of Ministry of Education of China [NCET-08-0905]
- National Department Public Benefit Research Foundation (Agriculture) of China [2010003020]
- Scientific Research Foundations of the Education Department of Sichuan Province, China [09zz026]
This study investigated the effects of myo-inositol (MI) on the growth and antioxidant capacity of carp enterocytes. The enterocytes were incubated in media containing 0, 15, 30, 45, 60 and 75 mg MI L-1 for 96 h. The results indicated that MI could increase cell viability. In addition, the activities of cellular alkaline phosphatase (AKP), gamma-glutamyl transpeptidase (?-GT), Na+, K+-adenosine trisphosphatase (Na+, K+-ATPase) and creatinkinase (CK) increased with MI supplementation at levels ranging from 15 to 60 mg MI L-1 medium, indicating an improvement in cell differentiation and function. Further, enzymatic antioxidant ability, as measured by total superoxide dismutase (T-SOD), Cu/Zn-SOD, Mn-SOD, catalase (CAT), glutathione peroxidase (GPx) and glutathione-S-transferase (GST) activities, improved with MI supplementation. Finally, cell damage, as indicated by lactic acid dehydrogenase (LDH) activity, malondialdehyde (MDA) content of the medium and cellular protein carbonyls (PC), was all depressed by MI. Correlation analyses showed that cell viability (MTT) was positively related to the antioxidant enzyme activities, but negatively related to cell damage (LDH, MDA and PC). In summary, the data showed that MI could improve the growth of fish enterocytes. This result may be partly due to the enhanced antioxidant status and depressed oxidative damage.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据