期刊
JOURNAL OF CELL SCIENCE
卷 117, 期 13, 页码 2813-2825出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.01136
关键词
ryanodine receptor; pacemaker; calcium oscillation; c-Kit-immunopositive cells; interstitial cells of Cajal
类别
Intracellular Ca2+ ([Ca2+](i)) oscillations seen in interstitial cells of Cajal (ICCs) are considered to be the primary pacemaker activity in the gut. Here, we show evidence that periodic Ca2+ release from intracellular Ca2+ stores produces [Ca2+](i) oscillations in ICCs, using cell cluster preparations isolated from mouse ileum. The pacemaker [Ca2+](i) oscillations in ICCs are preserved in the presence of dihydropyridine Ca2+ antagonists, which suppress Ca2+ activity in smooth muscle cells. However, applications of drugs affecting either ryanodine receptors or inositol 1,4,5-trisphosphate receptors terminated [Ca2+](i) oscillations at relatively low concentrations. RT-PCR analyses revealed a predominant expression of type 3 RyR (RyR3) in isolated c-Kit-immunopositive cells (ICCs). Furthermore, we demonstrate that pacemaker-like global [Ca2+](i) oscillation activity is endowed by introducing RyR3 into HEK293 cells, which originally express only IP(3)Rs. The reconstituted [Ca2+](i) oscillations in HEK293 cells possess essentially the same pharmacological characteristics as seen in ICCs. The results support the functional role of RyR3 in ICCs.
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