期刊
AQUACULTURE INTERNATIONAL
卷 22, 期 2, 页码 699-709出版社
SPRINGER
DOI: 10.1007/s10499-013-9699-5
关键词
16S rDNA; Antagonism; Artemia; Lactic acid bacteria; Vibrio
类别
资金
- EGIDE (CMCU project France-Tunisia) [08G 0907]
- Agence Universitaire de la Francophonie (AUF-PSCI) [6313PS809]
Lactic acid bacteria (LAB) were isolated from rotifer cultures in a marine hatchery to search for potential probiotics for marine animals. Fifteen strains were first selected among a total of 55, according to antibacterial activity against Vibrio sp. Among eight strains identified as Lactobacillus casei, four were highly adhesive, suggesting some ability for surface colonization. The other strains were identified as Lactobacillus plantarum, Lactobacillus dextrinicus, and Leuconostoc sp. To validate probiotic potential, Artemia were challenged against pathogenic Vibrio alginolyticus, with or without one of six selected LAB strains. The six strains protected Artemia against the pathogen, to some extent on condition that nutrient enrichment was provided. La. casei BR51 and X2 were preferred, as they were efficient even in the absence of nutrient supply. La. casei X2 was finally selected as candidate probiotic, due to the best growth performances of Artemia, with or without the pathogen.
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