期刊
JOURNAL OF IMMUNOLOGICAL METHODS
卷 289, 期 1-2, 页码 65-80出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2004.03.014
关键词
antibody engineering; phage display; human antibodies; functional screening
We introduce a procedure for the rapid generation of fully human antibodies derived from Fab-on-phage display libraries. The technology is based on the compatibility of display vectors and IgG expression constructs, and allows reformatting of individual Fab clones to IgG, as well as reformatting of antibody repertoires. Examples of batch reformatting of an uncharacterized Fab repertoire and of a pool of Fabs, previously analyzed at the phage level, are presented. The average transient expression levels of the IgG constructs in HEK293T cells are above 10 mug/ml, allowing the use of conditioned media in functional assays without antibody purification. Furthermore, we describe a high-throughput purification method yielding IgG amounts sufficient for initial antibody characterization. Our technology allows the generation and production of antigen-specific complete human antibodies as fast or even faster than raising monoclonal antibodies by conventional hybridoma techniques. (C) 2004 Elsevier B.V. All rights reserved.
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