期刊
AQUACULTURE
卷 434, 期 -, 页码 11-17出版社
ELSEVIER
DOI: 10.1016/j.aquaculture.2014.07.021
关键词
Swimming crab; Cold temperature; Antioxidant defense; Oxidative damage; Heat shock proteins; Apoptosis
资金
- National High Technology Research and Development Program of China [2012AA10A409]
- Projects of independent innovation in Shandong Province [2013CXC80202]
- Special Scientific Research Funds for Central Non-profit Institutes, Yellow Sea Fisheries Research Institutes [20603022013027]
Cold temperature can lead to large-scale mortality of overwinter cultured swimming crab Portunus trituberculatus; however, little is currently known about the physiology of this species under low temperature. The present study was carried out to investigate the effects of cold exposure on physiology of P. trituberculatus. Activities of antioxidant enzymes (SOD and CAT), oxidative damage to lipid and protein (indexed by contents of malondialdehyde (MDA) and protein carbonyl (PC), respectively), and expression of heat shock protein genes (hsp70, hsp90-1 and hsp90-2) and a marker of apoptosis, caspase-3, in muscle and hepatopancreas tissues of the crabs acclimated at the temperatures of 3, 6, 9, 12, 15 and 22 degrees C (control) for four weeks were measured. SOD and CAT activities in the crabs increased significantly at 9, 12 and 15 degrees C, but decreased markedly at 3 and 6 degrees C, compared with those in control crabs. Accumulation of MDA and PC in both muscle and hepatopancreas tissues was observed when the crabs were acclimated at 3 and 6 degrees C. Abundance of hsp70, hsp90-1 and hsp90-2 mRNA increased with decreasing temperature, reached the maximum levels at 6 degrees C and then decreased sharply at 3 degrees C. Caspase-3 expression, in coincidence with MDA and PC contents, increased significantly at 3 and 6 degrees C. These results suggested that at low temperatures antioxidant defense system and heat shock proteins in swimming crabs are enhanced to prevent cellular damage and can effectively protect cells against apoptosis; however, cold stress beyond a critical level results in depression of the protective mechanisms, which in turn leads to cellular components damage, and elevated levels of apoptotic cell death. Based on the results of this study, overwintering P. trituberculatus should be cultured at temperatures above 6 degrees C. (C) 2014 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据