期刊
BIOLOGY OF REPRODUCTION
卷 70, 期 6, 页码 1545-1551出版社
OXFORD UNIV PRESS INC
DOI: 10.1095/biolreprod.103.021998
关键词
dopamine; gonadotropin-releasing hormone; gonadotropin-releasing hormone receptor; pituitary
The present work was designed to study certain aspects of the endocrine regulation of gonadotropin-releasing hormone receptor (GnRH-R) in the pituitary of the teleost fish tilapia. A GnRH-R was cloned from the pituitary of hybrid tilapia (taGnRH-R) and was identified as a typical seven-transmembrane receptor. Northern blot analysis revealed a single GnRH-R transcript in the pituitary of approximately 2.3 kilobases. The taGnRH-R mRNA levels were significantly higher in females than in males. Injection of the salmon GnRH analog (sGnRHa; 5-50 mug/kg) increased the steady-state levels of taGnRH-R mRNA, with the highest response recorded at 25 mug/kg and at 36 h. At the higher dose of sGnRHa (50 mug/kg), taGnRH-R transcript appeared to be down-regulated. Exposure of tilapia pituitary cells in culture to graded doses (0.1-100 nM) of seabream (sbGnRH = GnRH 1), chicken 11 (cGnRH 11), or salmon GnRH (sGnRH = GnRH 111) resulted in a significant increase in taGnRH-R mRNA levels. The highest levels of both LH release and taGnRH-R mRNA levels were recorded after exposure to cGnRH 11 and the lowest after exposure to sbGnRH. The dopamine-agonist quinpirole suppressed LH release and mRNA levels of taGnRH-R, indicating an inhibitory effect on GnRH-R synthesis. Collectively, these data provide evidence that GnRH in tilapia can up-regulate, whereas dopamine down-regulates, taGnRH-R mRNA levels.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据