期刊
PLANT MOLECULAR BIOLOGY REPORTER
卷 22, 期 2, 页码 179-188出版社
SPRINGER
DOI: 10.1007/BF02772725
关键词
copy number; puroindoline-b; real-time PCR; transgene; wheat
Transgene copy number is usually determined by means of Southern blot analysis, which can be time consuming and laborious. In this study, quantitative real-time PCR was developed to determine transgene copy number in transgenic wheat. A conserved wheat housekeeping gene, puroindoline-b, was used as an internal control to calculate transgene copy number. Estimated copy number in transgenic lines using real-time quantitative PCR was correlated with actual copy number based on Southern blot analysis. Real-time PCR can analyze hundreds of samples in a day, making it an efficient method for estimating copy number in transgenic wheat.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据