期刊
AQUACULTURE
卷 312, 期 1-4, 页码 212-216出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aquaculture.2011.01.005
关键词
Brazilian aquaculture; Shrimp virus; Viral co-infection; Molecular diagnostic; IHHNV; IMNV; qPCR
资金
- National Brazilian Council for the Scientific and Technological Development (CNPq), Ministry of Science and Technology [578460/08-4]
- Ministry of Fishery and Aquiculture, Federal Government of Brazil
- CNPq
Cultivation of whiteleg shrimp (Litopenaeus vannamei) constitutes a growing aquaculture industry in northeast Brazil. Similar to other animals that are intensively farmed, shrimp experience disease outbreaks, which are a constant threat and cause eventually significant economical losses. Infectious hypodermal and hematopoietic necrosis virus (IHHNV) and infectious myonecrosis virus (IMNV) are prevalent epizootic viral agents in Brazil. In a routine monitoring program for the diagnosis of IHHNV and IMNV, using molecular techniques like conventional PCR, reverse transcription coupled with PCR (RT-PCR) and absolute quantitative PCR (qPCR), we found that most positive samples of shrimp were simultaneously co-infected with both viruses. This survey is the first to show the occurrence of a natural co-infection that is caused by IHHNV and IMNV in penaeid shrimp attacked by viral disease that were cultivated in Northeast Brazil. (C) 2011 Elsevier B.V. All rights reserved.
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