期刊
AQUACULTURE
卷 315, 期 3-4, 页码 213-221出版社
ELSEVIER
DOI: 10.1016/j.aquaculture.2011.02.034
关键词
Scallop Chlamys farreri; Haemocyte; beta-glucan; Enzyme activity; Water temperature
资金
- NSFC [30901112]
- National 863 Project [2006AA100307]
- Agriculture Ministry [200803012-04]
High water temperatures are associated with immune suppression and elevated mortality in economically important marine bivalves. To determine whether the stimulatory effects of beta-glucan on enzymes associated with the immune system were depressed at higher temperatures, scallops Chlamys farreri were treated with 0.5, 1.0 or 2.0 mg/ml of beta-glucan suspensions at 15 degrees C or 25 degrees C, and the activities of six enzymes ( phenoloxidase (PO), acid phosphatase (ACP), superoxide dismutase (SOD), peroxidase (POD), myeloperoxidase (MPO) and alkaline phosphatase (ALP)) were measured in haemocytes every 12 h for seven days. The results showed that at 15 degrees C, compared to the control, the 0.5 mg/ml treatment resulted in significant induction of PO activity, the 1.0 mg/ml treatment stimulated PO, ACP, SOD, POD and MPO activities, and the 2.0 mg/ml treatment stimulated PO, ACP, SOD, POD and ALP activities (p<0.05). At 25 degrees C, significant activation of PO activity was observed with the 0.5 mg/ml treatment, while PO, ACP and SOD activities were induced by the 1.0 mg/ml treatment and ACP activity by the 2.0 mg/ml treatment (p<0.05); however, no significant induction of POD or MPO activity was observed. Surprisingly, ALP activity was undetectable at 25 degrees C throughout the entire experimental period. These results indicate that the 1.0 mg/ml beta-glucan treatment consistently had the highest stimulatory effect among the three concentrations. PO, ACP and SOD were more sensitive than POD. MPO and ALP to beta-glucan or temperature stress. The extent and duration of activation as well as the time of peak emergence for all six enzymes appeared higher, longer and earlier at 15 degrees C than 25 degrees C in all beta-glucan treatments, suggesting that high temperature may weaken or delay immune reactions to exogenous stress. (C) 2011 Elsevier B.V. All rights reserved.
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