期刊
PLANT AND CELL PHYSIOLOGY
卷 45, 期 6, 页码 667-671出版社
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pch087
关键词
cultivation; Cyanidioschyzon merolae; 5-FOA resistance; homologous recombination; red algae; transformation
Although the nuclear genome sequence of Cyanidioschyzon merolae 10D, a unicellular red alga, was recently determined, DNA transformation technology that is important as a model plant system has never been available thus far. In this study, improved culture conditions resulted in a faster growth rate of C. merolae in liquid medium (doubling time = 9.2 h), and colony formation on gellan gum plates. Using these conditions, spontaneous mutants (5-fluoroortic acid resistant) deficient in the UMP synthase gene were isolated. The lesions were then restored by introducing the wild-type UMP synthase gene into the cells suggesting DNA transformation by homologous recombination.
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