期刊
GENES & GENETIC SYSTEMS
卷 79, 期 3, 页码 139-144出版社
GENETICS SOC JAPAN
DOI: 10.1266/ggs.79.139
关键词
Arabidopsis thaliana; CENP-C; centromere; 180-bp repeat; immuno fluorescence
Centromere protein C (CENP-C) is a component of the kinetochore essential for correct segregation of sister chromatids in mammals. In Arabidopsis thaliana, a single-copy gene encoding a protein homologous to CENP-C has been found by homology in the whole-genome sequence. To investigate the CENP-C homolog (AtCENP-C), we cloned cDNAs by RT-PCR and determined its full-length coding sequence. Antibodies against the synthetic peptide for the C-terminal residues of AtCENP-C detected a polypeptide in Arabidopsis cell extracts on western blots. Immunofluorescence labeling with the antibodies and fluorescence in situ hybridization demonstrated clearly that AtCENP-C is present at the centromeric regions throughout the cell cycle.
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