期刊
AQUACULTURE
卷 294, 期 3-4, 页码 202-211出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aquaculture.2009.06.020
关键词
Turbot Mgp; Gene expression; Soft tissue accumulation; Heart; Vertebrae; Branchial arches
资金
- FCT (Portuguese Science and Technology Foundation) [FISHDEV POCri/CVr/42098/2001]
- POCI 2010
- FEDER
- MCYT (Spanish Ministerio de Ciencia y Tecnologia) [MCY-r/AG12003-03558]
Matrix Gla protein (Mgp) is a secreted vitamin K-dependent extracellular matrix protein and a physiological inhibitor of calcification whose gene structure, amino acid sequence and tissue distribution have been conserved throughout evolution. In the present work. the turbot (Scophthalmus maximus) mgp cDNA was cloned and the sequence of the deduced protein compared to that of other vertebrates. As expected, it was closer to teleosts than to other vertebrate groups but there was a strict conservation of amino-acids thought to be important for protein function. Analysis of mgp gene expression indicated branchial arches as the site with higher levels of expression, followed by heart, vertebra and kidney. These results were confirmed by in situ hybridization with a strong mgp expression in branchial arch chondrocytes. Mgp was found to accumulate in gills where it appeared to be restricted to chondrocytes from branchial filaments, while in vertebrae it was localized in vertebral end plates, in growth zones, in vertebral arches and spines and in notochord cells. In the soft tissues analysed, Mgp was mainly detected in kidney and heart, consistent with previous data and providing further evidence for a role of Mgp as a calcification inhibitor and a modulator of the mineralization process. Our studies provide evidence that turbot, an important new species for aquaculture, is also a useful model to study function and expression of Mgp. (c) 2009 Elsevier B.V. All rights reserved.
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