期刊
MOLECULAR ECOLOGY NOTES
卷 4, 期 2, 页码 262-264出版社
BLACKWELL PUBLISHING LTD
DOI: 10.1111/j.1471-8286.2004.00636.x
关键词
chloroplast; grasses; microsatellite; Poaceae; simple sequence repeat; universal primers
Attempts to design truly universal primers to amplify chloroplast microsatellites have met with limited success due to nonconservation of repeat loci across widely divergent taxa. We have used the complete chloroplast genome sequences of rice, maize and wheat to design five pairs of primers that amplify homologous mononucleotide repeats across the Poaceae (grasses). Sequencing confirmed conservation of repeat motifs across subfamilies and a preliminary study in Anthoxanthum odoratum revealed polymorphism at two loci with a haplotype diversity value of 0.495. These primers provide a valuable tool to study cytoplasmic diversity in this extensively studied and economically important range of taxa.
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