期刊
AQUACULTURE
卷 284, 期 1-4, 页码 35-40出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aquaculture.2008.07.034
关键词
Streptococcus agalactiae; Tilapia; Genotyping; Pathogenicity
资金
- Thailand Research Fund
- The Royal Golden jubilee
- Bureau of the Budget, Thailand
Streptococcus agalactiae (Group B streptococcus, GBS) is an important pathogen affecting humans and animals as well as aquatic species. Between April 2003 and March 2006, bacteria were isolated from infected red tilapia Oreochromis sp. and Nile tilapia O. niloticus cultured in Thailand. They were identified as CBS by API 20 STREP, polymerase chain reaction (PCR) and multiplex PCR-based reverse line blot hybridization (mPCR/RLB). Genotyping indicated that they belonged to two genotypes. One of these genotypes has not been identified in any of 1792 GBS isolates genotyped by mPCR/RLB; it belongs to serotype la and contains genes encoding proteins C alpha (bca) and C beta (bac), three insertions sequences (IS 1381, IS861 and ISSag2) and the group It intron GBSi1. The other has recently been identified among invasive human isolates in Hong Kong; it belongs to an uncommon subtype of serotype III (III-4), contains bca, three insertion sequences (IS1381, ISSag1 and ISSag2) and a tetracycline resistance gene (tetM). Pathogenicity studies in red tilapia by experimental infection established that CBS is a pathogen of fish. Histopathological changes indicated that infection of tilapia with GBS produced disease with systemic involvement characterized by multiple necrotic foci in various tissues. The possible epidemiology and epizoology of these bacteria among human, mammal and aquatic species are discussed in this report. (C) 2008 Elsevier B.V. All rights reserved.
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