期刊
EUROPEAN JOURNAL OF IMMUNOLOGY
卷 34, 期 6, 页码 1542-1550出版社
WILEY
DOI: 10.1002/eji.200324586
关键词
leishmania; Langerhans cells; parasitic protozoan; antigen presentation
类别
In the early phase of leishmaniasis three types of potential antigen-presenting cells, including epidermal Langerhans cells (LC), dermal dendritic cells (DC) and inflammatory DC, are localized at the site of infection. Therefore, it has been a central question which cell type is responsible for the initiation of a protective immune response. In the early stage of an anti-Leishmania immune response, detectable Leishmania major antigen was localized in the paracortex of the draining lymph nodes (LN). Characterization of antigen-positive cells showed that L. major co-localized with DC of a CD11c(+) CD8alpha(-) Langerin(-) phenotype. To determine the area of antigen uptake, dermis or epidermis, and to further define the type of antigen-transporting cells, L. major was inoculated subcutaneously and concurrently LC were mobilized with fluorescein isothiocyanate (FITC). After 3 days, DC carrying L. major antigen were always FITC-, indicating a dermal and not an epidermal origin. Moreover, addition of L. major antigen to ex vivo isolated CD8alpha(-) and CD8alpha(+) DC from the draining LN of L. major-infected C57BL/6 mice demonstrated that both DC subpopulations were able to stimulate antigen-specific T cell proliferation in vitro. Without addition of exogenous antigen only the CD8a- Langerin DC were capable of stimulating antigen-specific T cell proliferation. Thus, we demonstrate that CD8alpha(-) Langerin(-) DC and not LC are the basis of the protective immune response to intracellular L. major parasites in vivo.
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