Mossbauer- and EPR-snapshots of an enzymatic reaction:: The cytochrome P450 reaction cycle

In this communication we present a complimentary Mossbauer- and EPR-study of the time dependance of the reaction of substrate free P450cam with peracetic acid within a time region ranging from 8 ms up to 5 min. An Fe(IV) species as well as a tyrosyl radical residing on the amino acid residue Tyr96 have been identified as reaction intermediates. These species possibly are formed by the reduction of compound I by means of transferring an electron from Tyr96 to the heme moiety.