期刊
EMBO REPORTS
卷 5, 期 6, 页码 626-631出版社
WILEY
DOI: 10.1038/sj.embor.7400154
关键词
Sgt1; Hec1; Mad2; CENP; kinetochore; spindle checkpoint
资金
- NCI NIH HHS [P30 CA021765, CA21765] Funding Source: Medline
- NIGMS NIH HHS [R01 GM068418, GM68418] Funding Source: Medline
- Wellcome Trust [073915] Funding Source: Medline
Budding yeast Sgt1 is required for kinetochore assembly, and its homologues have a role in cAMP signalling in fungi and pathogen resistance in plants. The function of mammalian Sgt1 is unknown. We report that RNA interference-mediated depletion of Sgt1 from HeLa cells causes dramatic alterations of the mitotic spindle and problems in chromosome alignment. Cells lacking Sgt1 undergo a mitotic delay due to activation of the spindle checkpoint. The checkpoint response, however, is significantly weakened in Sgt1-depleted cells, and this correlates with a dramatic reduction in kinetochore levels of Mad1, Mad2 and BubR1. These effects are explained by a problem in kinetochore assembly that prevents the localization of Hec1, CENP-E, CENP-F, CENP-I, but not CENP-C, to mitotic kinetochores. Our studies implicate Sgt1 as an essential protein and a critical assembly factor for the mammalian kinetochore, and lend credit to the hypothesis of a kinetochore assembly pathway that is conserved from yeast to man.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据