期刊
DNA SEQUENCE
卷 15, 期 3, 页码 225-227出版社
INFORMA HEALTHCARE
DOI: 10.1080/10425170410001723949
关键词
cloning vector; cosmid; RK2; broad host range
This paper presents the complete sequencing and annotation of the pLAFR1 vector. pLAFR is a tetracycline-resistant cosmid cloning vector, which is derived from the 20 kb plasmid pRK290, a RK2-derivative. Due to its broad host range, the pLAFR1 vector has been widely used in the genetic analysis of a broad number of gram-negative bacterial species. The availability of the complete pLAFR1 sequence will most definitely help in the construction and analysis of clone libraries based on pRK290 or pLAFR vectors.
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