期刊
JOURNAL OF CELL BIOLOGY
卷 165, 期 5, 页码 673-683出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200402130
关键词
PACT domain; centrosome; centriole; Drosophila; mitosis
类别
资金
- NIDCD NIH HHS [R01 DC002780, DC02780] Funding Source: Medline
- Wellcome Trust Funding Source: Medline
Centrosomes consist of a pair of centrioles surrounded by an amorphous pericentriolar material (PCM). Proteins that contain a Pericentrin/AKAP450 centrosomal targeting (PACT) domain have been implicated in recruiting several proteins to the PCM. We show that the only PACT domain protein in Drosophila (the Drosophila pericentrin-like protein [D-PLP]) is associated with both the centrioles and the PCM, and is essential for the efficient centrosomal recruitment of all six PCM components that we tested. Surprisingly, however, all six PCM components are eventually recruited to centrosomes during mitosis in d-plp mutant cells, and mitosis is not dramatically perturbed. Although viable, d-plp mutant flies are severely uncoordinated, a phenotype usually associated with defects in mechanosensory neuron function. We show that the sensory cilia of these neurons are malformed and the neurons are nonfunctional in d-plp mutants. Moreover, the flagella in mutant sperm are nonmotile. Thus, D-PLP is essential for the formation of functional cilia and flagella in flies.
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