期刊
APPLIED SURFACE SCIENCE
卷 231, 期 -, 页码 452-456出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.apsusc.2004.03.170
关键词
prostate cancer; ToF-SIMS; Fr-IR microspectroscopy; cell lines; imaging
Imaging ToF-SIMS and synchrotron-based Fourier transform infrared (SR-FT-IR) microspectroscopy have been used to obtain chemical information from individual cells derived from human prostate cancer (CaP). ToF-SIMS imaging of molecular signals characteristic of membrane bound phospholipids are used to elucidate different fracture planes within individual freeze-fractured CaP cells. The localisation of Cu within the cytoplasm of cancer cells is consistent with increased metastatic potential. Line scans across CaP cells using SR-FT-IR micro spectroscopy provide complimentary information on the localisation (+/-1 mum) of lipid and protein domains. This combined analytical approach offers a novel means of characterising individual CaP cells and investigating the biochemical basis of disease progression and metastases. (C) 2004 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据