Fluorescence and terbium-sensitised luminescence determination of garenoxacin in human urine and serum

Fluorescence and terbium-sensitised luminescence properties of new quinolone garenoxacin have been studied. The fluorimetric method allows the determination of 0.060-0.600 mug ml(-1) of garenoxacin in aqueous solution containing HCl/KCl buffer (pH 1.5) with lambda(exc) = 282 nm and lambda(em) = 421 nm. Micellar-enhanced fluorescence was also studied, leading to a higher than 400% increase in analytical signal in presence of 12 mM sodium dodecyl sulphate (SIDS), allowing the determination of 0.020-0.750 mug ml(-1) of garenoxacin. The terbium-sensitised luminescence method allows the determination of 0.100-1.500 mug ml(-1) of garenoxacin in 12 mM SIDS solution containing 0.08 M acetic acid/sodium acetate buffer (pH 4.1) and 7.5 mM Na2SO3 (chemical deoxygenation agent), with lambda(exc) = 281 nm and lambda(em) = 546 nm. Relative standard deviation (R.S.D.) values for the three methods were in the range 1.0-2.0%. The proposed procedures have been applied to the determination of garenoxacin in spiked human urine and serum. (C) 2004 Elsevier B.V. All rights reserved.