期刊
MOLECULAR IMMUNOLOGY
卷 41, 期 5, 页码 527-538出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.molimm.2004.03.034
关键词
IgG; immunoglobulin; variable region; Fab; domain exchange; multimerization; structure; function
资金
- NIAID NIH HHS [AI029470, AI039187] Funding Source: Medline
Variable region domain exchanged IgG, or inside-out (io), molecules, were produced to investigate the effects of domain interactions on antibody structure and function. Studies using ultracentrifugation and electron microscopy showed that variable region domain exchange induces non-covalent multimerization through Fab domains. Surprisingly, variable region exchange also affected Fc-associated functions such as serum half-life and binding to protein G and FcgammaRI. These alterations were not merely a consequence of IgG aggregation. Both the extent of multimerization and alterations in Fc-associated properties depended on the IgG isotype. (C) 2004 Elsevier Ltd. All rights reserved.
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