期刊
OSTEOARTHRITIS AND CARTILAGE
卷 12, 期 7, 页码 559-567出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.joca.2004.04.001
关键词
autologous chondrocyte transplantation; tissue engineering; alginate cultures; BMP
Objective: For autologous chondrocyte transplantation (ACT) chondrocytes are expanded in vitro. During expansion these cells may dedifferentiate. This change in phenotype is characterized by a raised expression of type I collagen and a decrease in type 11 collagen expression. Since high expression of type 11 collagen is of central importance for the properties of hyaline cartilage, we investigated if the growth factor bone morphogenetic protein-2 (BMP-2) may modulate the chondrogenic phenotype in monolayer cell cultures and in three-dimensional culture systems. Design: Chondrocytes from articular knee cartilage of 11 individuals (average age: 39.8 years) with no history of joint disease were isolated and seeded either in monolayer cultures or embedded in alginate beads in presence or absence of human recombinant BMP-2 (hr-BMP-2). Then, cells were harvested and analysis of the chondrogenic phenotype was performed using quantitative RT-PCR, immunocytochemistry and ELISA. Results: Addition of BMP-2 to chondrocytes expanded in two-dimensional (2D) cultures during the first subculture (P1) had no effect on mRNA amounts encoding type 11 collagen and interleukin-1beta(IL-1beta). In contrast, seeding chondrocytes in three-dimensional (3D) alginate cultures raised type 11 collagen expression significantly and addition of BMP-2 enhanced this effect. Conclusions: We conclude that chondrocytes during expansion for ACT may benefit from BMP-2 activation only when seeded in an appropriate 3D culture system. (C) 2004 OsteoArthritis Research Society International. Published by Elsevier Ltd. All rights reserved.
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