期刊
JOURNAL OF BACTERIOLOGY
卷 186, 期 14, 页码 4829-4833出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.186.14.4829-4833.2004
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We disrupted the reverse gyrase gene from a hyperthermophilic archaeon, Thermococcus kodakaraensis KOD1 An apparent positive supercoiling activity that was observed in the host strain was not found in the disruptant strain. We found that a lack of reverse gyrase led to a retardation in growth that was more striking at higher temperatures. However, the disruption of the reverse gyrase gene did not lead to a lethal phenotype at 90degreesC. This study provides experimental evidence that reverse gyrase is not a prerequisite for hyperthermophilic life.
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