期刊
NUCLEIC ACIDS RESEARCH
卷 32, 期 -, 页码 W340-W345出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkh447
关键词
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资金
- NIGMS NIH HHS [R01 GM067193] Funding Source: Medline
ProSight PTM (https://prosightptm.scs.uiuc.edu/) is a web application for identification and characterization of proteins using mass spectra data from 'top-down' fragmentation of intact protein ions (i.e. without any tryptic digestion). ProSight PTM has many tools and graphical features to facilitate analysis of single proteins, proteins in mixtures and proteins fragmented in parallel. Sequence databases from across the phylogenetic tree are supported, with a new database strategy of 'shotgun annotation' used to assist characterization of wild-type proteins. During a database search, data from divergent sources regarding potential mass differences such as polymorphisms, alternate splicing and post-translational modifications are utilized. The user can optionally control how much of this biological variability should be searched.
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