期刊
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
卷 122, 期 1, 页码 20-27出版社
OXFORD UNIV PRESS INC
DOI: 10.1309/529T2WDNEB6X8VUN
关键词
immuno-PCR; IPCR; polymerase chain reaction; p24 antigen; HIV-1; viral load; ELISA; enzyme-linked immunosorbent assay; real-time PCR
类别
Presently, the assay that attains maximal sensitivity and dynamic range of HIV-1 viral copy number (50 copies per milliliter) is nucleic acid amplification of HIV RNA in plasma. Enzyme-linked immunosorbent assay (ELISA) methods for quantification of HIV-1 p24 antigen have been relatively insensitive. In this report, we show data that indicate real-time immuno polymerase chain reaction (IPCR), a combination of the ELISA and PCR techniques, is more sensitive for HIV-1 p24 antigen detection than other currently reported methods. When derived from an IPCR standard curve, a dose response was observed from patient samples with known viral loads diluted within a 3-log range (1.68-6,514 viral RNA copies per milliliter). IPCR detected 42% (22/52) of patient samples that had fewer than 50 viral RNA copies per milliliter by reverse transcriptase-PCR. IPCR shows the potential to become the most analytically sensitive test available for determination of HIV-1 viral load by the detection of HIV-1 p24 antigen.
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