期刊
JOURNAL OF SYNCHROTRON RADIATION
卷 11, 期 -, 页码 314-318出版社
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S0909049504014086
关键词
SAXS; liquid chromatography; Guinier; aggregation
资金
- NCRR NIH HHS [RR-08630] Funding Source: Medline
Small-angle X-ray scattering and size-exclusion chromatography have been combined within a unified experimental set-up to obtain molecular size information. Besides providing simultaneous corroborative data bearing on the same question from two distinct experimental techniques, passing the samples over a gel filtration column immediately prior to illumination by X-rays provides both a more homogeneous sample and a continuous set of data as the concentration is extrapolated to zero. This greatly facilitates analysis of data from oligomerizing or aggregating proteins and increases the reliability of the results.
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