期刊
BIOMEDICAL CHROMATOGRAPHY
卷 18, 期 6, 页码 396-399出版社
WILEY
DOI: 10.1002/bmc.346
关键词
capillary electrochromatography; barbiturates; benzodiazepines; 3-(1,8-naphthalimido)propyl-modified silyl; silica gel
A capillary electrochromatographic (CEC) method was applied to the simultaneous separation of barbiturates (barbital, phenobarbital, secobarbital and thiopental) and benzodiazepines (nitrazepam, diazepam and triazolam). The separation was performed in a 75 pm i.d. capillary, packed with 3-(1,8-naphthalimido)propyl-modified silyl silica gel (NAIP), studying the effects of buffer pH and mobile phase composition. Using an applied voltage of 20 kV and the short-end injection method (9 cm capillary effective length), the mobile phase of 1.0 mm citrate buffer (pH 5.0) containing 45% methanol provided the baseline separation of seven toxic drugs in less than 9 min. In CEC with NAIP, the benzodiazepines were separated by the combination of hydrophobic and pi-pi interactions, whereas the separation of the barbiturates was based on the hydrophobic interaction. Copyright (C) 2004 John Wiley Sons, Ltd.
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