期刊
APPLIED SURFACE SCIENCE
卷 276, 期 -, 页码 717-722出版社
ELSEVIER
DOI: 10.1016/j.apsusc.2013.03.159
关键词
Bacterial flagella; Biotemplate; Salmonella typhimurium; Silver nanoparticle; Bionanomaterials; Characterization
The present study was carried out to synthesize one dimensional silver nanoparticle impregnated flagellar bionanomaterial. Flagella was isolated from Salmonella typhimurium and depolymerised into flagellin monomers. The flagellin monomers were repolymerised again into flagella using suitable technique. The molecular weight of native (NF) and polymerized flagella (PF) was determined using polyacrylamide gel electrophoresis. The NF and PF were used as a template, over which silver nanoparticles were impregnated using in situ chemical reduction process. The synthesized flagellar-silver nanoparticle bionanomaterials were characterized using UV-vis, FT-IR Raman and XRD spectroscopy, and High resolution transmission electron microscopy (HR-TEM). The characterization studies confirmed the attachment of silver nanoparticles over flagella and repolymerised flagella. The size of the silver nanoparticles on the flagella and repolymerised flagella varied and was in the range of 3-11 nm. I-V characteristics of the bionanomaterials were analyzed using Kethley meter which indicated the increase of conductivity after impregnation of silver nanoparticles. The results indicated that flagellar-silver nanoparticle bionanomaterials can be used as a potential one dimensional bionanomaterials for various applications. (c) 2013 Elsevier B.V. All rights reserved.
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