期刊
NATURE STRUCTURAL & MOLECULAR BIOLOGY
卷 11, 期 7, 页码 650-655出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nsmb786
关键词
-
The histone variant H2A. Z plays an essential role in metazoans but its function remains to be determined. Here, we developed a new inducible RNAi strategy to elucidate the role of H2A. Z in mammalian cell lines. We show that in the absence of H2A. Z, the genome becomes highly unstable and that this instability is caused by defects in the chromosome segregation process. Analysis of H2A. Z localization reveals that in these cells it is enriched at heterochromatic foci with HP1alpha on the arms of chromosomes but not at centromeric regions. When H2A. Z is depleted, normal HP1alpha-chromatin interactions are disrupted on the chromosomal arms and, notably, also at pericentric regions. Therefore, H2A. Z controls the localization of HP1alpha. We conclude that H2A. Z is essential for the accurate transmission of chromosomes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据