期刊
JOURNAL OF PROTEOME RESEARCH
卷 3, 期 4, 页码 834-840出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr049941r
关键词
copper-binding proteins; Hep G2; proteomics; peroxiredoxin
To further our knowledge of intracellular copper transport, we used a proteomics strategy to search for hepatic proteins with copper-binding ability. Hep G2 cytosolic and microsomal fractions were applied to a copper(II)-loaded immobilized metal-affinity chromatography (IMAC) column. Protein identification was performed with 2-D gel electrophoresis and mass spectrometry. We identified 48 cytosolic proteins and 19 microsomal proteins displaying copper-binding ability. These proteins are diverse in function. Fifty-two of the 67 proteins contain putative metal-binding domains. We have identified many components of the Hep G2 copper metalloproteome including a large number of proteins not previously known to bind copper.
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