期刊
APPLIED SURFACE SCIENCE
卷 259, 期 -, 页码 201-207出版社
ELSEVIER
DOI: 10.1016/j.apsusc.2012.07.019
关键词
Magnetite nanoparticle; Surface modification; Gene delivery
类别
资金
- National Natural Science Foundation of China (NSFC) [20876100, 20736004]
- National Basic Research Program of China (973 Program) [2009CB219904]
- Key Laboratory of Organic Synthesis of Jiangsu Province
- Post-doctoral Science Foundation of Jiangsu Province
- National Post-doctoral Science Foundation [20090451176]
- Commission of Science and Technology of Suzhou Municipality [YJS0917, SG0978]
- Technology Innovation Foundation of Suzhou New District & MOST [11C26223204581]
- Natural Science Foundation of Jiangsu Province [BK2011328]
- Minjiang Scholarship of Fujian Province
The PEI-CMD-MNPs were successfully prepared by the surface modification of magnetic Fe3O4 nanoparticles with carboxymethyl dextran (CMD) and polyethyleneimine (PEI). The PEI-CMD-MNPs polyplexes exhibited a typical superparamagnetic behavior and were well stable over the entire range of pH and NaCl concentration. These PEI-CMD-MNPs were used as magnetic gene vectors for targeting gene delivery. The prepared MNPs at different surface modification stages were characterized using Fourier transform infrared (FT-IR), thermogravimetric analysis (TGA), field emissions canning electron microscopy (FE-SEM), powder X-ray diffraction (XRD) and dynamic laser light scattering (DLS) analysis. The magnetic properties were studied by vibrating sample magnetometer (VSM). To evaluate the performance of the magnetic nanoparticles as gene transfer vector, the PEI-CMD-MNPs were used to delivery green fluorescent protein (GFP) gene into BHK21 cells. The expression of GFP gene was detected by fluorescence microscope. DNA-PEI-CMD-MNPs polyplexes absorbed by the cells were also monitored by Magnetic resonance imaging (MRI). The transfection efficiency and gene expression efficiency of that transfected with a magnet were much higher than that of standard transfection. (C) 2012 Elsevier B.V. All rights reserved.
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