期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 70, 期 7, 页码 4411-4414出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.70.7.4411-4414.2004
关键词
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The recently developed CARD-FISH protocol was refined for the detection of marine Archaea by replacing the lysozyme permeabilization treatment with proteinase K. This modification resulted in about twofold-higher detection rates for Archaea in deep waters. Using this method in combination with microautoradiography, we found that Archaea are more abundant than Bacteria (42% versus 32% of 4',6'-diamidino-2-phenylindole counts) in the deep waters of the North Atlantic and that a larger fraction of Archaea than of Bacteria takes up L-aspartic acid (19% versus 10%).
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