期刊
APPLIED SURFACE SCIENCE
卷 258, 期 10, 页码 4322-4327出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.apsusc.2011.12.107
关键词
Titanium; Ammonia; Plasma immersion ion implantation; MC3T3-E1 cells
类别
资金
- National Natural Science Foundation of China [30700170, 30973041]
We report here a new method of titanium surface modification through ammonia (NH3) plasma immersion ion implantation (Pill) technique and its effect on the cellular behaviors of MC3T3-E1 osteoblastic cells. The NH3 PIII-treated titanium substrates (NH3-Ti) were characterized by X-ray photoelectron (XPS), which showed that NH3-Ti had a nitrogen-rich surface. However, there was no significant difference between the surface morphology of NH3-Ti and unmodified Ti. When MC3T3-E1 cells were cultured on NH3-Ti substrates, it was found that cell proliferation was accelerated at 4 and 7 days of culture. Meanwhile, cell differentiation was evaluated using type I collagen (COL I), osteocalcin (OC) and bone sialoprotein (BSP) as differentiation markers. It was found that expression of COL I and OC genes was up-regulated on NH3-Ti substrates. However, no significant difference was found in BSP gene expression between NH3-Ti and unmodified Ti substrates. Therefore, findings from this study indicate that surface modification of titanium through NH3 PIII favors osteoblastic proliferation and differentiation and as a result, it may be used to improve the biocompatibility of Ti implants in vivo. (C) 2011 Elsevier B.V. All rights reserved.
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