期刊
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
卷 52, 期 7, 页码 985-989出版社
SAGE PUBLICATIONS LTD
DOI: 10.1369/jhc.4B6271.2004
关键词
extrarenal vitamin D synthesis; colorectal cancer; CYP27B1 (25-hydroxyvitamin D-3-1 alpha-hydroxylase); vitamin D receptor; immunofluorescence; real-time PCR; differentiated colon tumor cells
类别
1,25-dihydroxyvitamin D-3 has anti-mitotic, pro-differentiating, and proapoptotic activity in tumor cells. We demonstrated that the secosteroid can be synthesized and degraded not only in the kidney but also extrarenally in intestinal cells. Evaluation of 1,25-dihydroxyvitamin D-3-synthesizing CYP27131 hydroxylase mRNA (real-time PCR) and protein (immunoblotting, immunofluorescence) showed enhanced expression in high- to medium-differentiated human colon tumors compared with tumor-adjacent normal mucosa or with colon mucosa from non-cancer patients. In high-grade undifferentiated tumor areas expression was lost. Many cells co-expressed CYP27131 and the vitamin D receptor. We suggest that autocrine/paracrine antimitotic activity of 1,25-dihydroxyvitamin D3 could prevent intestinal tumor formation and progression.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据