期刊
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
卷 130, 期 7, 页码 423-428出版社
SPRINGER
DOI: 10.1007/s00432-004-0556-9
关键词
ovarian neoplasm; cisplatin (cis-diamine-dichloro-platinum); chemoresistance; apoptosis; caspase-3; Bcl-2
类别
Goals. Resistance to cisplatin is the main reason for treatment failure in ovarian cancer. Apoptosis is the main mechanism of action of most cancer chemotherapeutic agents. The apoptosis-associated proteins expressed in cisplatin-sensitive (A2780, COC1) and -resistant (A2780/DDP, COC1/DDP) ovarian cancer cell lines, as well as their effects on caspase-3 activity in these cells, were studied by reverse transcriptase polymerase chain reaction and Western blot analysis. Methods. The apoptotic ratios of A2780, COC1, A2780/DDP, and COC1/DDP cells after treatment with cisplatin were measured by flow cytometry. Results. Expression of Bcl-2 and Bcl-X-L in A2780/DDP and COC1/DDP cells was significantly higher than that in A2780 and COC1 cells, respectively. Expression of Bax and Bcl-Xs did not differ in cisplatin-resistant and -sensitive cells. Caspase-3 activity was reduced markedly and apoptotic ratios were significantly lower in A2780/DDP and COC1/DDP cells than in A2780 and COC1 cells after treatment with cisplatin. Conclusion. We conclude that overexpression of antiapoptotic proteins Bcl-2 and Bcl-X-L and down-regulation of caspase-3 activity may be associated with cisplatin resistance in human ovarian cancer.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据