期刊
GENE EXPRESSION PATTERNS
卷 4, 期 4, 页码 361-370出版社
ELSEVIER
DOI: 10.1016/j.modgep.2004.01.014
关键词
zebrafish; neuropilin; soluble neuropilin; duplicate genes; zebrafish linkage groups; vascular endothelial growth factor; blood vessels; brain
资金
- NCI NIH HHS [CA37392, CA45448] Funding Source: Medline
- NIDDK NIH HHS [DK49216] Funding Source: Medline
- PHS HHS [H148801] Funding Source: Medline
Previously, we described the isolation and characterization of the first zebrafish neuropilin gene, which we now call nrp1a, and found its protein to be a mediator of vascular endothelial growth factor (VEGF)-dependent angiogenesis [Proc. Natl Acad. Sci. USA 99 (2002) 10470]. Subsequently, we have isolated three other full-length neuropilin genes (nrp1b, nrp2a, and nrp2b) and find that they map to independent zebrafish linkage groups. The nrp1s and nrp2s had differential spatio-temporal gene expression profiles with nrp1a being most prominent in the gut, brain, retina, hypochord, motorneurons, fin bud and mandibular cartilage, nrplb in the brain, dorsal aorta, melanophores, ventral fin, and heart, nrp2a in the brain, retina, heart, and caudal vessels, and nrp2b in the brain, retina, gut, fin bud, melanophores, heart, and caudal vessels. In addition, we have identified an alternatively-spliced transcript of the nrplb gene (denoted as nrp1b(s)) which is predicted to encode a soluble form of Nrp1b, containing only the a, b, and c extracellular domains. Transcript expression of nrplb(s) was different from full-length nrp1b transcript, with prominence in the brain, developing mouth, heart, and fin bud. The NRP1s were tested for VEGF-binding ability. Both 125 kDa Nrp1a and 145 kDa Nrp1b bound I-125-labelled VEGFA(165). In summary, two nrp1 and two nrp2 genes, with expression patterns similar to higher vertebrates, have been isolated from zebrafish. (C) 2004 Elsevier B.V. All rights reserved.
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