期刊
APPLIED SURFACE SCIENCE
卷 262, 期 -, 页码 8-12出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.apsusc.2011.12.009
关键词
Arrangement technique; Osteoblast-like cell; Antibody protein; Mineralization; beta-Sheet peptide template; Amorphous calcium phosphate
类别
资金
- Japan Society for the Promotion of Science (JSPS) [23560925]
- SER program from the Institute of Ceramics Research and Education (ICRE)
- Grants-in-Aid for Scientific Research [23560925] Funding Source: KAKEN
We demonstrate arrangement techniques of proteins and cells using an amorphous calcium phosphate (ACP) nanofiber scaffold. It is well known that protein andosteoblastic cell are preferably adsorbed onto ACP surface. The ACP nanofiber scaffold was prepared by calcium phosphate mineralization on a polypeptide monolayer-coated mica substrate, and ACP nanofibers were oriented unidirectionaly. In a protein system, the ACP nanofiber scaffold was soaked in a fluorescein isothiocyanate conjugated immunoglobulin G (IgG-FITC) aqueous solution. From fluorescence microscopic measurement, the adsorbed IgG-FITC was highly confined and arranged on the ACP nanofiber. In a cell system, a mouse osteoblast-like cell (MC3T3-E1) behavior on the ACP nanofiber scaffold was observed. The cell was elongated unidirectionaly, and its cytoskeletal shape showed high aspect ratio. These results are clearly different from an ACP bulk template or bare mica substrate, and the arrangement technique enable to fabricate a fine-tuned biomaterial template. Crown Copyright (c) 2011 Published by Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据