期刊
ANALYTICA CHIMICA ACTA
卷 515, 期 1, 页码 127-141出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2003.09.044
关键词
gradient elutions; high-performance liquid chromatography; off-line optimisation; evolutionary algorithm
A new system for the unattended optimisation of gradient elutions in reversed-phase high-performance liquid chromatography has been developed. The system is based on the simulation of retention times under conditions of ternary solvent mixtures and ternary gradient programmes. This model is constructed departing from a few experimental data obtained in isocratic elutions and validated against experimental gradient elutions. Once validated, this retention model can be used for the unattended search of an optimum separation. The optimisation process is driven by an evolutionary algorithm (EA), specially developed to map the ternary gradient elutions problem. The development and characteristics of the retention modelling as well as those of the EA and its particularities are discussed and some real world examples of separation presented. (C) 2003 Elsevier B.V. All rights reserved.
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