Caste determination in a polyembryonic wasp involves inheritance of germ cells

Social insects are characterized by the development of castes in which some colony members reproduce whereas others function as altruistic helpers. The conditional switch controlling caste formation usually involves environmental stimuli that act on processes that regulate development of individuals. Unlike other social species, embryos of polyembryonic wasps develop clonally to produce large numbers of genetically identical offspring and two morphologically distinct castes. All embryos in a clone exist in an identical environment, the host, yet develop into either reproductive larvae that mature into adult wasps or soldier larvae whose function is defense. Here, we report that caste determination in Copidosoma floridanum involves inheritance of germ cells. Expression of a C. floridanum homolog (Cf-vas) of the germ cell marker Vasa indicated that the B-4 blastomere in four cell-stage embryos is specified as a primordial germ cell. Vas expression later in development further indicated that embryos developing into reproductive larvae possess primordial germ cells whereas embryos developing into soldier larvae do not. Ablation of the B-4 blastomere resulted in most broods containing only soldiers whereas ablation of other blastomeres produced broods containing both castes. These results indicate that soldier larvae are obligately sterile and reveal a previously unknown role for germ cells in caste formation.