期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 101, 期 3, 页码 308-315出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2004.03.015
关键词
Love-wave biosensor array; aptamer; thrombin; Rev
A Love-wave biosensor array has been designed by coupling aptamers to the surface of a Love-wave sensorchip. The sensor chip consists of five single sensor elements and allows label-free, real-time, and quantitative measurements of protein and nucleic acid binding events in concentration-dependent fashion. The biosensor was calibrated for human alpha-thrombin and HIV-1 Rev peptide by binding fluorescently labelled molecules and correlating the mass of the bound molecules to fluorescence intensity. Detection limits of approximately 75 pg/cm(2) were obtained, and analyte recognition was specific. The sensor can easily be regenerated by simple washing steps. We further demonstrate the versatile applicability of the sensor by immobilizing single-stranded DNA (ssDNA) for the detection of the corresponding counter-strand. (C) 2004 Elsevier B.V. All rights reserved.
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