期刊
CARBOHYDRATE POLYMERS
卷 56, 期 4, 页码 423-427出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2004.03.008
关键词
extracellular polysaccharide; Pseudomonas caryophylli; purification; characterization; structure
Extracellular polysaccharides were isolated from Pseudomonas caryophylli CFR 1705 grown on lactose containing medium. The major fraction (no.1) obtained on DEAE-cellulose chromatography was composed of rhamnose, mannose and glucose in the ratio 1:3.26:4.97, respectively, and having a molecular weight of 1.1x10(6) Da. Methylation followed by GC-MS analysis revealed it to be a highly branched 1,4-linked hexosan with mannose and glucose as the branch-off residues at positions C-2 and C-6 of the main chain. Rhamnose was essentially found as non-reducing terminal residue. (C) 2004 Elsevier Ltd. All rights reserved.
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