期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 279, 期 29, 页码 30600-30610出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M400710200
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资金
- NIGMS NIH HHS [GM32134] Funding Source: Medline
The four-component toluene/o-xylene monooxygenase (ToMO) from Pseudomonas stutzeri OX1 is capable of oxidizing arenes, alkenes, and haloalkanes at a carboxylate-bridged diiron center similar to that of soluble methane monooxygenase (sMMO). The remarkable variety of substrates accommodated by ToMO invites applications ranging from bioremediation to the regio- and enantiospecific oxidation of hydrocarbons on an industrial scale. We report here the crystal structures of the ToMO hydroxylase (ToMOH), azido ToMOH, and ToMOH containing the product analogue 4-bromophenol to 2.3 or greater resolution. The catalytic diiron( III) core resembles that of the sMMO hydroxylase, but aspects of the alpha(2)beta(2)gamma(2) tertiary structure are notably different. Of particular interest is a 6-10 Angstrom-wide channel of similar to35 Angstrom in length extending from the active site to the protein surface. The presence of three bromophenol molecules in this space confirms this route as a pathway for substrate entrance and product egress. An analysis of the ToMOH active site cavity offers insights into the different substrate specificities of multicomponent monooxygenases and explains the behavior of mutant forms of homologous enzymes described in the literature.
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