期刊
JOURNAL OF CELL BIOLOGY
卷 166, 期 2, 页码 179-191出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200404001
关键词
mitosis; aurora B kinase; survivin; INCENP; TD-60
类别
资金
- Wellcome Trust [073915] Funding Source: Medline
The chromosomal passenger complex of Aurora B kinase, INCENP, and Survivin has essential regulatory roles at centromeres and the central spindle in mitosis. Here, we describe Borealin, a novel member of the complex. Approximately half of Aurora B in mitotic cells is complexed with INCENP, Borealin, and Survivin; and Borealin binds Survivin and INCENP in vitro. A second complex contains Aurora B and INCENP, but no Borealin or Survivin. Depletion of Borealin by RNA interference delays mitotic progression and results in kinetochore-spindle misattachments and an increase in bipolar spindles associated with ectopic asters. The extra poles, which apparently form after chromosomes achieve a bipolar orientation, severely disrupt the partitioning of chromosomes in anaphase. Borealin depletion has little effect on histone H3 serine(10) phosphorylation. These results implicate the chromosomal passenger holocomplex in the maintenance of spindle integrity and suggest that histone H3 serine(10) phosphorylation is performed by an Aurora B-INCENP subcomplex.
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