期刊
FEBS LETTERS
卷 571, 期 1-3, 页码 107-111出版社
WILEY
DOI: 10.1016/j.febslet.2004.05.086
关键词
endocytosis; exocytosis; chromogranin; calcineurin
资金
- NIDDK NIH HHS [R01 DK 57999] Funding Source: Medline
Exocytic fusion in neuroendocrine cells does not always result in complete release of the peptide contents from dense core vesicles (DCVs). In this study, we use fluorescence imaging and immunoelectron microscopy to examine the retention, endocytosis and recycling of chromogranin B in DCVs of NGF-treated PC12 cells. Our results indicate that DCVs retained and retrieved an intact core that was available for subsequent exocytic release. The endocytic process was inhibited by cyclosporine A or by substitution of extracellular Ca2+ with Ba2+, and the total recycling time was less than 5 min. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
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