期刊
PLOS BIOLOGY
卷 2, 期 8, 页码 1224-1232出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pbio.0020233
关键词
-
资金
- NIAID NIH HHS [R01-AI34867, R01 AI034867] Funding Source: Medline
- NIGMS NIH HHS [R01-GM064625, R01 GM064625] Funding Source: Medline
Synaptotagmin is considered a calcium-dependent trigger for regulated exocytosis. We examined the role of synaptotagmin VII (Syt VII) in the calcium-dependent exocytosis of individual lysosomes in wild-type (WT) and Syt VII knockout (KO) mouse embryonic fibroblasts (MEFs) using total internal reflection fluorescence microscopy. In WT MEFs, most lysosomes only partially released their contents, their membrane proteins did not diffuse into the plasma membrane, and inner diameters of their fusion pores were smaller than 30 nm. In Syt VII KO MEFs, not only was lysosomal exocytosis triggered by calcium, but all of these restrictions on fusion were also removed. These observations indicate that Syt VII does not function as the calcium-dependent trigger for lysosomal exocytosis. Instead, it restricts the kinetics and extent of calcium-dependent lysosomal fusion.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据