期刊
PLANT AND CELL PHYSIOLOGY
卷 45, 期 8, 页码 1099-1104出版社
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pch117
关键词
Arabidopsis thaliana; nuclear magnetic resonance; plant metabolomics; stable isotope labeling
Nuclear magnetic resonance (NMR) will become a key technology in plant metabolomics with the use of stable isotope labeling and advanced hetero-nuclear NMR methodologies. To demonstrate the power of this approach, we performed multi-dimensional hetero-nuclear NMR analysis of metabolic movement of carbon and nitrogen nuclei in Arabidopsis thaliana. First, distinct ethanol-stress response was investigated using C-13-labeled wild type and an ethanol-hypersensitive mutant plants. Furthermore, we followed nitrogen fluxes in N-15-labeled seeds during the initiation of germination in vivo. The future role of stable isotope-labeling combined with advanced hetero-nuclear NMR in plant metabolomics is discussed.
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